Liquid Culture to Agar Filled Petri Dish
Date:
Goal
The goal is to transfer liquid culture onto an agar filled petri dish in order to provide the mycelium with a sterile and nutrient rich enviroment that will aid and speed up growth as well as allow the mycologist to choose a segment with the strongest mycelium to ensure success.
Materials Required
- Petri Dishes fill with Agar
- Liquid Culture or Spore syringe
- Rubbing Alcohol
- Still Air box
- Gloves
- Lighter
- Paper Towels
Method
1. Gather equpment
2. Heat oven 150 degrees Celsius (Farenheight)
4 Wash and dry petri dishes
5. Wrap 10 petri dishes in foil
6. Place wrapped petri dishes in oven
Observations and Results
Observations
Results
Conclusion
My method of pouring and inoculating was subpar because I haven’t done agar work in a while
I had more contaminations than normal and that could be because of
- Knocking over the petri dishes when adding the parafilm
- Leaving the petri dishes (without parafilm) in the still air box for 3 days
- Not shaking the liquid culture syringe properly